Using the Gap Analysis problem-solving framework (Clark & Estes, 2008), this project examined engineering students at Abu Dhabi Colleges at Higher Colleges of Technology (HCT) in the United Arab Emirates. The primary purpose of this project was to identify the knowledge, motivation and organizational causes that prevent graduates from the HCT bachelor of applied science in engineering program from pursuing a masters degree in engineering in order to become faculty. Mixed methods were used to collect survey data from 493 participants and interview data for twenty participants to identify and validate the knowledge and skills, motivation, and organizational solutions that may contribute to the HCT s Emiratisation goal. Findings showed that although students lack factual knowledge, students do not lack motivation in pursuing a master degree. However, the study confirmed their lack of interest in working in an educational environment or pursuing a career as faculty, and it confirmed organizational gap in HCT management. Based on the findings, solutions are offered to address these challenges.
Worldwide, a number of research groups are functioning towards effective treatment of colon cancer using chemotherapeutic agents. Nonsteroidal anti-inflammatory drugs are among the most potent agents discovered for the inhibition of cancer. In spite of the approval of celecoxib for adjuvant therapy in patients with familial adenomatous polyposis and precancerous disease of colon, associations of larger intensity of side effects limit its usage in cancer therapy. Combination therapy provides advantages of reduction in dose and possible reduction in toxicity and acquired drug resistance. As a consequence, targeted drug delivery and targeted molecular therapy of single or combination of anticancer agents are necessary for efficient treatment of colon cancer with reduced toxicity. In our study, combination of celecoxib and AEE788 shows growth inhibition and apoptosis in HCT 15 cells. Further, nanocarrier mediated celecoxib delivery showed high entrapment efficiency, sustained release patterns, desirable hemocompatibility and enhanced cytotoxicity and apoptosis in vitro and in vivo.
The emergence of extensive antibiotics resistant bacteria increased the demands for find out new source of antimicrobial agent, Actinomycetes, especially Streptomycetes spp have recently grasped considerable attention worldwide due to production of many useful bioactive metabolites that exploited by the pharmaceutical industry. In present study, a number of 52 Actinomycetes were recovered, from which 20 showed antimicrobial activities to at least one tested organism. The most potent isolate AGM12-1, which was active against all tested organisms and with IC50 3.3 and 1.1mig/ml. against HCT 116 and HepG-2 respectively was characterized as Streptomyces vinaceusdrappus with presence of ARO-PKS II genes. Mannitol, ammonium sulfate, pH 7, 2% stating inoculums and incubation for 11 days at 30°c were the optimum conditions used to maximize the production and hence allowed purification of one active compound to homogeneity using RP-HPLC with m/z 488.05.The NMR structural elucidation showed that this compound was a derivative of Diketopiperazine.
HIV&AIDS Counselling and Testing (HCT) services have for long been seen as an intervention towards HIV prevention. The major objective of this study is to establish the Knowledge and Attitudes of youth towards HCT services in Lira district, Uganda. The study tried to establish the knowledge of the youth on HIV&AIDS and HCT services, to identify the attitudes of the youth towards HCT services and to find out the strategies for improving HCT uptake among the youth. A cross section study design was used to collect quantitative and qualitative data from 112 youth. Quantitative data was collected by the use of semi-structure questionnaire on youth between 15-24 years while qualitative data was obtained using key informant interviews and focus group discussions. The findings show that most youth are knowledgeable about HIV&AIDS and HCT services because they can identify modes of HIV transmission and where to obtain HCT services. Youth in school were more likely to have heard about HIV and AIDS compared to the youth out of school. The study shows that youth have both positive and negative attitudes towards HCT services.
Recombinant technology has crucial impact in therapy development. In microbial environment, Pathogenic bacteria such as Staphylococcus aureus produces alpha hemolysin protein. This protein is used as anticancer protein. alpha hemolysin gene was isolated from pathogenic S. aureus that was isolated from blood samples of patients (chrildren). This recombinant hia gene was produced alpha hemolysin protein by using competent E. coli as a host. The recombinant - toxin protein was used as anticancer agent.In this study that high concentration of -toxin (500 mil/ ml) was effected on the cell viability of four cells line ( HepG-2, HCT-116, MCF-7, A-549). The cell viability of cell lines were represented 31.69% in HepG-2, 36.27% in HCT-116, 78.95% in MCF-7, 67.28% in A-549. From our results, Alpha hemolysin protein had high inhibitory activity against HepG-2 and HCT-116, and had weak inhibitory activity against MCF-7 and A-549. Inconclusion,The production of recombinant - toxin was very simple process, low cost, high efficiency, non-toxic, high yield and no side effect.
Recent advances in the field of RNA interference (RNAi) have enabled researchers to conduct in depth investigations of gene function using RNAi libraries. Our second-generation shRNA libraries consist of over 200,000 short hairpin RNA constructs targeting over 45,000 human and mouse genes modeled after primary miRNA transcripts. Each hairpin is linked to a unique 60 nucleotide identification sequence, which serves as a barcode and allows us to virtually count the number of cells that contain a specific hairpin in a cell population. Small changes in barcode copy number can be monitored through the use of microarray technology. The barcode can be amplified from a cell's genomic DNA and fluorescently labeled to produce a probe that is hybridized to a microarray. I applied this RNAi barcode screening method in a screen using a complex mixture of 7500 library hairpins in a p53 isogenic HCT 116 colon cancer cells to identify genes that modify sensitivity to a common chemotherapeutic, doxorubicin. This work illustrates the powerful use of RNAi screens to search for genes that synergize with existing therapeutics, and suggests strategies for genetically informed combination therapies.
The book aimed to reveal degradation of Cucurbitacin E glucoside obtained from Citrullus colocynthis (L.) (Hanzal) into Cucurbitacin E and glucose by mean of microbial biotransformation using Curvularia lunata NRRL 2178 as a source of beta-glucosidase. All the instrumental data obtained by highly advanced techniques, confirmed the microbial conversion of cucurbitacin E glucoside into cucurbitacin E and glucose indicating effect of enzyme in the hydrolysis process by splitting of glucose from the anomeric carbon atom. The effect of the cucurbitacin E glucoside and cucurbitacin E on the proliferation of Hep-G2 cells was studied. The treatment of Hep-G2 cells with cucurbitacin E led to a high inhibition of the cell proliferation, which revealed a moderate anti-tumor activity of the cucurbitacin E against hepatic carcinoma, while cucurbitacin E glucoside had no cytotoxic effect on Hep-G2 cells. The study showed the effect of the compounds on the proliferation of HCT-116 cells, the two compounds were not cytotoxic as indicated by their IC50 values. On the other hand, the treatment of T-lymphocyte cells with cucurbitacin E resulted in increase in the cell proliferation.
Master Thesis in Applied Chemical Science, it Includes studing 27 different olive tree cultivars leaves ethanolic extracts in terms of their total phenolic content, antioxidant activity, toxicity to brine shrimps, anticancer activity (cell cycle analysis, cell death assay, MTT test), safety on normal cells (MTT test), LC-MS anlysis and quntification for some of their chemical constituents like Oleuropein, Oleanolic acid, Erythrodiol with its isomer Uvaol, Quercetin, Rutin. investigation of the presence of Resveratrol and Salicin qualitatively and quantitatively using LC-MS anlysis , studing the correlation between each constituent concentration in the extracts and their toxicity to brine shrimps also their correlation with anticancer activity on 7 cancer cell lines (MCF- 7, MDA-MB-231, C32, MV3, SW480, SW620, HCT-116), LC- MS(-)ESI,(+)APCI finger print of the most potent anticancer extracts.
The title of my book is Assessment of Hematological Parameters in Ischemic Stroke Patients. The study was done at hospital from January 2013 to July 2013. For this purpose samples were collected from local population and divided into two groups i.e. Ischemic and controlled. Data regarding risk factors was collected by Questionnaire. Their hematological parameters such as WBCs count, RBCs count, Hgb concentration, HCT, MCV, MCH and PLT count were assessed by hematology analyzer. Statistical analysis was done by using SPSS (Version 13). The WBCs count, MCV, MCH and HCT were found significantly higher in ischemic stroke subjects while significant decrease was observed in RBCs count and hemoglobin concentration. PLT count increased in ischemic stroke subjects. It is concluded that in ischemic stroke patients hematological parameters are greatly altered.