Phytic acid is extensively known for its anti-nutritional properties. The wide notion about this compound is to be changed totally. Hence, the study is focused on bringing out its potential as a drug molecule. The knowledge gained by understanding the binding efficacy of phytic acid and its analogues can help in the development of a new lead molecule. The antibacterial studies and cell line studies using HCT-15 confirmed the drugability. The potency of cancer drug targets can also be screened using docking studies. The entire work can bring out an effective lead molecule which maybe subjected to clinical trials. It is hypothesized that phytic acid and its derivatives having one or more of the following chemical functional groups such as the number of phosphates, phospho-diesters, charged phosphates, halogens, heavy atoms, steriocentred atoms, hydoxyl groups, hydrogen donors and acceptors, have an effect on the binding affinity towards the selected targets. Although, there are few reports to suggest the anticancer activity of PA, there is no work on its structure-activity relationship.
Recent advances in the field of RNA interference (RNAi) have enabled researchers to conduct in depth investigations of gene function using RNAi libraries. Our second-generation shRNA libraries consist of over 200,000 short hairpin RNA constructs targeting over 45,000 human and mouse genes modeled after primary miRNA transcripts. Each hairpin is linked to a unique 60 nucleotide identification sequence, which serves as a barcode and allows us to virtually count the number of cells that contain a specific hairpin in a cell population. Small changes in barcode copy number can be monitored through the use of microarray technology. The barcode can be amplified from a cell's genomic DNA and fluorescently labeled to produce a probe that is hybridized to a microarray. I applied this RNAi barcode screening method in a screen using a complex mixture of 7500 library hairpins in a p53 isogenic HCT 116 colon cancer cells to identify genes that modify sensitivity to a common chemotherapeutic, doxorubicin. This work illustrates the powerful use of RNAi screens to search for genes that synergize with existing therapeutics, and suggests strategies for genetically informed combination therapies.
Healing properties of propolis are known in folk medicine from antiquity, however, recently, the interest in propolis as a potential natural product is increasing because of its broad spectrum of biological properties. It is an anti-flammatory agent, an immunostimulant, anti-oxidant, anti-microbial, and it is also an anti-tumour and a carcinostatic agent. This study was undertaken to characterize the chemical compounds, and antioxidant and anti-tumour potentials of propolis samples collected from five different geographical locations in Iraq using in vitro and in vivo studies. Thirty-eight different compounds were identified, and clerodane diterpenoids were identified for first time in temperate zone. All propolis samples exhibited strong free radical scavenging activity. The in vitro evaluations by growth inhibition, clonogenicity and flow cytometric assays showed that propolis has cytotoxic effects on two human cell lines. The in vivo potentials were also recorded on the growth of HCT-116 xenografts in a nude mouse model. This study provides the rationale to investigate the potential beneficial effect of propolis in the diet of patients receiving anti-cancer therapies.
Master Thesis in Applied Chemical Science, it Includes studing 27 different olive tree cultivars leaves ethanolic extracts in terms of their total phenolic content, antioxidant activity, toxicity to brine shrimps, anticancer activity (cell cycle analysis, cell death assay, MTT test), safety on normal cells (MTT test), LC-MS anlysis and quntification for some of their chemical constituents like Oleuropein, Oleanolic acid, Erythrodiol with its isomer Uvaol, Quercetin, Rutin. investigation of the presence of Resveratrol and Salicin qualitatively and quantitatively using LC-MS anlysis , studing the correlation between each constituent concentration in the extracts and their toxicity to brine shrimps also their correlation with anticancer activity on 7 cancer cell lines (MCF- 7, MDA-MB-231, C32, MV3, SW480, SW620, HCT-116), LC- MS(-)ESI,(+)APCI finger print of the most potent anticancer extracts.
This research work has attempted to investigate the underlying determinant factors of being HIV positive or HIV negative from the available HCT data using data mining techniques. It consists of experiments on searching classification model that predicts HIV status and association rule mining to discover the relationship of HIV status with the selected attributes. The classification experiments are carried out using J48 and ID3 algorithms. The association rule mining is using Apriori algorithm. One of the surprising results obtained from the experiment was that age group 50 and above are becoming also vulnerable to HIV/AIDS as the patterns have indicated. Medical experts also have stated that a growing number of older people are being infected with HIV/AIDS. One of the reasons they raised is that, they are finding HIV more often than ever before in older age since improved treatments are helping people with the disease live longer. The second reason is doctors do not always test older people for HIV/AIDS and so may miss some cases during routine check-ups and others.
The book aimed to reveal degradation of Cucurbitacin E glucoside obtained from Citrullus colocynthis (L.) (Hanzal) into Cucurbitacin E and glucose by mean of microbial biotransformation using Curvularia lunata NRRL 2178 as a source of beta-glucosidase. All the instrumental data obtained by highly advanced techniques, confirmed the microbial conversion of cucurbitacin E glucoside into cucurbitacin E and glucose indicating effect of enzyme in the hydrolysis process by splitting of glucose from the anomeric carbon atom. The effect of the cucurbitacin E glucoside and cucurbitacin E on the proliferation of Hep-G2 cells was studied. The treatment of Hep-G2 cells with cucurbitacin E led to a high inhibition of the cell proliferation, which revealed a moderate anti-tumor activity of the cucurbitacin E against hepatic carcinoma, while cucurbitacin E glucoside had no cytotoxic effect on Hep-G2 cells. The study showed the effect of the compounds on the proliferation of HCT-116 cells, the two compounds were not cytotoxic as indicated by their IC50 values. On the other hand, the treatment of T-lymphocyte cells with cucurbitacin E resulted in increase in the cell proliferation.
The title of my book is Assessment of Hematological Parameters in Ischemic Stroke Patients. The study was done at hospital from January 2013 to July 2013. For this purpose samples were collected from local population and divided into two groups i.e. Ischemic and controlled. Data regarding risk factors was collected by Questionnaire. Their hematological parameters such as WBCs count, RBCs count, Hgb concentration, HCT, MCV, MCH and PLT count were assessed by hematology analyzer. Statistical analysis was done by using SPSS (Version 13). The WBCs count, MCV, MCH and HCT were found significantly higher in ischemic stroke subjects while significant decrease was observed in RBCs count and hemoglobin concentration. PLT count increased in ischemic stroke subjects. It is concluded that in ischemic stroke patients hematological parameters are greatly altered.
Worldwide, a number of research groups are functioning towards effective treatment of colon cancer using chemotherapeutic agents. Nonsteroidal anti-inflammatory drugs are among the most potent agents discovered for the inhibition of cancer. In spite of the approval of celecoxib for adjuvant therapy in patients with familial adenomatous polyposis and precancerous disease of colon, associations of larger intensity of side effects limit its usage in cancer therapy. Combination therapy provides advantages of reduction in dose and possible reduction in toxicity and acquired drug resistance. As a consequence, targeted drug delivery and targeted molecular therapy of single or combination of anticancer agents are necessary for efficient treatment of colon cancer with reduced toxicity. In our study, combination of celecoxib and AEE788 shows growth inhibition and apoptosis in HCT 15 cells. Further, nanocarrier mediated celecoxib delivery showed high entrapment efficiency, sustained release patterns, desirable hemocompatibility and enhanced cytotoxicity and apoptosis in vitro and in vivo.
Using the Gap Analysis problem-solving framework (Clark & Estes, 2008), this project examined engineering students at Abu Dhabi Colleges at Higher Colleges of Technology (HCT) in the United Arab Emirates. The primary purpose of this project was to identify the knowledge, motivation and organizational causes that prevent graduates from the HCT bachelor of applied science in engineering program from pursuing a masters degree in engineering in order to become faculty. Mixed methods were used to collect survey data from 493 participants and interview data for twenty participants to identify and validate the knowledge and skills, motivation, and organizational solutions that may contribute to the HCT s Emiratisation goal. Findings showed that although students lack factual knowledge, students do not lack motivation in pursuing a master degree. However, the study confirmed their lack of interest in working in an educational environment or pursuing a career as faculty, and it confirmed organizational gap in HCT management. Based on the findings, solutions are offered to address these challenges.