Angebote zu "Microbial" (5 Treffer)

Kategorien

Shops

Microbial Production of Some Products of Cucurb...
61,90 € *
ggf. zzgl. Versand

The book aimed to reveal degradation of Cucurbitacin E glucoside obtained from Citrullus colocynthis (L.) (Hanzal) into Cucurbitacin E and glucose by mean of microbial biotransformation using Curvularia lunata NRRL 2178 as a source of beta-glucosidase. All the instrumental data obtained by highly advanced techniques, confirmed the microbial conversion of cucurbitacin E glucoside into cucurbitacin E and glucose indicating effect of enzyme in the hydrolysis process by splitting of glucose from the anomeric carbon atom. The effect of the cucurbitacin E glucoside and cucurbitacin E on the proliferation of Hep-G2 cells was studied. The treatment of Hep-G2 cells with cucurbitacin E led to a high inhibition of the cell proliferation, which revealed a moderate anti-tumor activity of the cucurbitacin E against hepatic carcinoma, while cucurbitacin E glucoside had no cytotoxic effect on Hep-G2 cells. The study showed the effect of the compounds on the proliferation of HCT-116 cells, the two compounds were not cytotoxic as indicated by their IC50 values. On the other hand, the treatment of T-lymphocyte cells with cucurbitacin E resulted in increase in the cell proliferation.

Anbieter: Dodax
Stand: 02.04.2020
Zum Angebot
Molecular Cloning of Staphylococcus Aureus Alph...
49,90 € *
ggf. zzgl. Versand

Recombinant technology has crucial impact in therapy development. In microbial environment, Pathogenic bacteria such as Staphylococcus aureus produces alpha hemolysin protein. This protein is used as anticancer protein. alpha hemolysin gene was isolated from pathogenic S. aureus that was isolated from blood samples of patients (chrildren). This recombinant hia gene was produced alpha hemolysin protein by using competent E. coli as a host. The recombinant - toxin protein was used as anticancer agent.In this study that high concentration of -toxin (500 mil/ ml) was effected on the cell viability of four cells line ( HepG-2, HCT-116, MCF-7, A-549). The cell viability of cell lines were represented 31.69% in HepG-2, 36.27% in HCT-116, 78.95% in MCF-7, 67.28% in A-549. From our results, Alpha hemolysin protein had high inhibitory activity against HepG-2 and HCT-116, and had weak inhibitory activity against MCF-7 and A-549. Inconclusion,The production of recombinant - toxin was very simple process, low cost, high efficiency, non-toxic, high yield and no side effect.

Anbieter: Dodax
Stand: 02.04.2020
Zum Angebot
Microbial Production of Some Products of Cucurb...
99,90 CHF *
ggf. zzgl. Versand

The book aimed to reveal degradation of Cucurbitacin E glucoside obtained from Citrullus colocynthis (L.) (Hanzal) into Cucurbitacin E and glucose by mean of microbial biotransformation using Curvularia lunata NRRL 2178 as a source of ss-glucosidase. All the instrumental data obtained by highly advanced techniques, confirmed the microbial conversion of cucurbitacin E glucoside into cucurbitacin E and glucose indicating effect of enzyme in the hydrolysis process by splitting of glucose from the anomeric carbon atom. The effect of the cucurbitacin E glucoside and cucurbitacin E on the proliferation of Hep-G2 cells was studied. The treatment of Hep-G2 cells with cucurbitacin E led to a high inhibition of the cell proliferation, which revealed a moderate anti-tumor activity of the cucurbitacin E against hepatic carcinoma, while cucurbitacin E glucoside had no cytotoxic effect on Hep-G2 cells. The study showed the effect of the compounds on the proliferation of HCT-116 cells; the two compounds were not cytotoxic as indicated by their IC50 values. On the other hand, the treatment of T-lymphocyte cells with cucurbitacin E resulted in increase in the cell proliferation.

Anbieter: Orell Fuessli CH
Stand: 02.04.2020
Zum Angebot
Microbial Production of Some Products of Cucurb...
54,99 € *
ggf. zzgl. Versand

The book aimed to reveal degradation of Cucurbitacin E glucoside obtained from Citrullus colocynthis (L.) (Hanzal) into Cucurbitacin E and glucose by mean of microbial biotransformation using Curvularia lunata NRRL 2178 as a source of ß-glucosidase. All the instrumental data obtained by highly advanced techniques, confirmed the microbial conversion of cucurbitacin E glucoside into cucurbitacin E and glucose indicating effect of enzyme in the hydrolysis process by splitting of glucose from the anomeric carbon atom. The effect of the cucurbitacin E glucoside and cucurbitacin E on the proliferation of Hep-G2 cells was studied. The treatment of Hep-G2 cells with cucurbitacin E led to a high inhibition of the cell proliferation, which revealed a moderate anti-tumor activity of the cucurbitacin E against hepatic carcinoma, while cucurbitacin E glucoside had no cytotoxic effect on Hep-G2 cells. The study showed the effect of the compounds on the proliferation of HCT-116 cells; the two compounds were not cytotoxic as indicated by their IC50 values. On the other hand, the treatment of T-lymphocyte cells with cucurbitacin E resulted in increase in the cell proliferation.

Anbieter: Thalia AT
Stand: 02.04.2020
Zum Angebot