Cancer is the leading cause of death in economically developed countries and the second leading cause of death in developing countries. Natural products are having a long history of use in the service of mankind for the prophylaxis and treatment of several diseases and cancer is not an exception.Triticum aestivum (Wheatgrass) has been traditionally used to treat various disorders and also used as a health tonic. 'WHOLE PLANTS FOR WHOLE PEOPLE' might be a suitable slogan to advertise the principles and practice of medical herbalism. In present study, aqueous extract, methanolic extract and isolated constituents were tested on various cancer cell lines like Hela, HEP- 3B, HCT- 15, HEp- 2 and the results were compared with the Vero cell line. Colchicine is taken as the reference standard.
Recent advances in the field of RNA interference (RNAi) have enabled researchers to conduct in depth investigations of gene function using RNAi libraries. Our second-generation shRNA libraries consist of over 200,000 short hairpin RNA constructs targeting over 45,000 human and mouse genes modeled after primary miRNA transcripts. Each hairpin is linked to a unique 60 nucleotide identification sequence, which serves as a barcode and allows us to virtually count the number of cells that contain a specific hairpin in a cell population. Small changes in barcode copy number can be monitored through the use of microarray technology. The barcode can be amplified from a cell's genomic DNA and fluorescently labeled to produce a probe that is hybridized to a microarray. I applied this RNAi barcode screening method in a screen using a complex mixture of 7500 library hairpins in a p53 isogenic HCT 116 colon cancer cells to identify genes that modify sensitivity to a common chemotherapeutic, doxorubicin. This work illustrates the powerful use of RNAi screens to search for genes that synergize with existing therapeutics, and suggests strategies for genetically informed combination therapies.
Healing properties of propolis are known in folk medicine from antiquity, however, recently, the interest in propolis as a potential natural product is increasing because of its broad spectrum of biological properties. It is an anti-flammatory agent, an immunostimulant, anti-oxidant, anti-microbial, and it is also an anti-tumour and a carcinostatic agent. This study was undertaken to characterize the chemical compounds, and antioxidant and anti-tumour potentials of propolis samples collected from five different geographical locations in Iraq using in vitro and in vivo studies. Thirty-eight different compounds were identified, and clerodane diterpenoids were identified for first time in temperate zone. All propolis samples exhibited strong free radical scavenging activity. The in vitro evaluations by growth inhibition, clonogenicity and flow cytometric assays showed that propolis has cytotoxic effects on two human cell lines. The in vivo potentials were also recorded on the growth of HCT-116 xenografts in a nude mouse model. This study provides the rationale to investigate the potential beneficial effect of propolis in the diet of patients receiving anti-cancer therapies.
Master Thesis in Applied Chemical Science, it Includes studing 27 different olive tree cultivars leaves ethanolic extracts in terms of their total phenolic content, antioxidant activity, toxicity to brine shrimps, anticancer activity (cell cycle analysis, cell death assay, MTT test), safety on normal cells (MTT test), LC-MS anlysis and quntification for some of their chemical constituents like Oleuropein, Oleanolic acid, Erythrodiol with its isomer Uvaol, Quercetin, Rutin. investigation of the presence of Resveratrol and Salicin qualitatively and quantitatively using LC-MS anlysis , studing the correlation between each constituent concentration in the extracts and their toxicity to brine shrimps also their correlation with anticancer activity on 7 cancer cell lines (MCF- 7, MDA-MB-231, C32, MV3, SW480, SW620, HCT-116), LC- MS(-)ESI,(+)APCI finger print of the most potent anticancer extracts.
Synthesis, Characterization, Anti-Proliferation, Benzimidazole Derivatives, FTIR, HRMS, 1D, 2D NMR spectroscopy, X ray crystallography, benzylation, addition/elimination, cyclization reactions, o- vanillin, o-, m-, p-phenylenediamines, 2-benzyloxy-3- methoxybenzaldehyde, 2-amino-N-benzylidene benzeneamines, bis-Schiff bases, six , five membered illusory rings, tetrahedral mechanism or addition/elimination mechanism, breast cancer cell line MCF 7, colon cancer cell line HCT 116, MTT assay, cytotoxicity, and IC50.
Cancer is one of the most dreaded diseases responsible for millions of deaths every year. In the present study, 12 medicinal plants from Jammu region, viz., Alstonia scholaris (devil tree), Azadirachta indica (neem), Calotropis gigantea (milk weed), Emblica officinalis (amla), Mentha citrata (bergamot mint), Mentha piperita (piper mint), Mentha viridis (pudina), Murraya koengii (kari pata), Musa paradisiaca (banana), Olea europaea (olive), Punica garanatum (pomegranate) and Trachyspermum ammi (ajwain) were evaluated against five human cancer cell lines from four different origins, viz., A-549 (lung), HCT-116 (colon), MCF-7 (breast), PC-3 (prostate) and T-47D (breast). 7 medicinal plants showed in vitro cytotoxic effect against one or the other human cancer cell line with the remarkable results produced by Calotropis gigantea (73-78% growth inhibition at 10 mig/ml against lung, colon and prostate cancer cells).To conclude, the plant can provide a great service and promise to cancer patients.
Worldwide, a number of research groups are functioning towards effective treatment of colon cancer using chemotherapeutic agents. Nonsteroidal anti-inflammatory drugs are among the most potent agents discovered for the inhibition of cancer. In spite of the approval of celecoxib for adjuvant therapy in patients with familial adenomatous polyposis and precancerous disease of colon, associations of larger intensity of side effects limit its usage in cancer therapy. Combination therapy provides advantages of reduction in dose and possible reduction in toxicity and acquired drug resistance. As a consequence, targeted drug delivery and targeted molecular therapy of single or combination of anticancer agents are necessary for efficient treatment of colon cancer with reduced toxicity. In our study, combination of celecoxib and AEE788 shows growth inhibition and apoptosis in HCT 15 cells. Further, nanocarrier mediated celecoxib delivery showed high entrapment efficiency, sustained release patterns, desirable hemocompatibility and enhanced cytotoxicity and apoptosis in vitro and in vivo.
This research work was carried out to establish scientific based evidence on folkloric usage and knowledge. The aim of this work is Pharmacological evaluation of Melochia corchorifolia leaves (folkloric) for antioxidant, diuretic, antiurolithiatic, anticancer, antibacterial and anthelmintic activities. In this regard, Melochia corchorifolia leaves were extracted with chloroform and ethylalcohol. The obtained extracts were used for pharmacological activities, the antioxidant activity results, the extracts and its Phenolic content shows good averting activity. Diuretic and saluretic activity is due to altering renal sodium excretion.The antiurolithiatic activity may be due to the presence of secondary metabolites of the extract.The extracts produced good anticancer activity on HCT-116 cell line and MCF-07 cancer cell lines. The chloroform extracts of Melochia corchorifolia shows good antibacterial and anthelmintic activities. So it was concluded that this research work supports the folkloric usage of this herbal drug (Melochia corchorifolia) is a potential source for treating various ailments.
A new Schiff base ligand (HL) derived from the condensation of quinoline-2-carboxaldehyde with 2-aminophenol and its mixed ligand complexes of Cr(III), Mn(II), Fe(III), Co(II), Ni(II), Cu(II), Zn(II) and Cd(II) vis 2,2'-bipyridine/1,10-phenanthroline as secondary ligand have been synthesized and characterized by elemental analyses, spectroscopic studies (IR, mass spectra, 1H NMR, UV-vis, magnetic susceptibility and solid reflectance), molar conductance, x-ray diffraction, ESR and thermal studies. The kinetic and thermodynamic parameters were calculated using the Coats-Redfern and HorowitzMetzger methods. Also, Schiff base ligand and its mixed ligand complexes were screened against Gram positive bacteria (Streptococcus pneumoniae and Bacillis subtilis) and Gram negative bacteria (Pseudomonas aeruginosa and Escherichia coli). Antifungal activity was carried out against (Aspergillus fumigatus and Candida albicans). In addition, anti-cancer activity of Schiff base ligand and its mixed ligand complexes were also tested against breast cancer cell line (MCF-7) and colon cancer cell line (HCT-116).