Recombinant technology has crucial impact in therapy development. In microbial environment, Pathogenic bacteria such as Staphylococcus aureus produces alpha hemolysin protein. This protein is used as anticancer protein. alpha hemolysin gene was isolated from pathogenic S. aureus that was isolated from blood samples of patients (chrildren). This recombinant hia gene was produced alpha hemolysin protein by using competent E. coli as a host. The recombinant - toxin protein was used as anticancer agent.In this study that high concentration of -toxin (500 mil/ ml) was effected on the cell viability of four cells line ( HepG-2, HCT-116, MCF-7, A-549). The cell viability of cell lines were represented 31.69% in HepG-2, 36.27% in HCT-116, 78.95% in MCF-7, 67.28% in A-549. From our results, Alpha hemolysin protein had high inhibitory activity against HepG-2 and HCT-116, and had weak inhibitory activity against MCF-7 and A-549. Inconclusion,The production of recombinant - toxin was very simple process, low cost, high efficiency, non-toxic, high yield and no side effect.
Scientific Study from the year 2012 in the subject Medicine - Gynecology, Andrology, Atlantic International University, course: Health Science, language: English, abstract: A total of 160 blood samples were collected into EDTA tubes between 1- January to 31-march 2011, at the Antenatal Clinic of Hospital of Porto Novo during the first visit of the pregnant women to the reproductive section and Using Sysmex KX-21-N by Sysmex corporation, Japan. The haematological parameters of Haemoglobin (Hb), Haematocrit (HCT), Mean Cell Volume (MCV) and Red Cell Distribution Width in coefficient variation (RDW-CV) were determined in all the samples; the trimester stages of each pregnancy were noted. Out of the 160 pregnant women blood samples analyzed, 62(38.8%) were anaemic, and the majority 71% were mildly anaemic cases, whereas 29% were moderately anaemic and no severe anaemic cases were recorded. Iron deficiency anaemia was 8.1% whereas 91.9% showed mixed anaemia indicating Normocytic- Normochromic. Anaemia was higher (53.2%) in pregnant women in the second trimester. CONCLUSION: The administration of iron with folic acid (folicferro), vitaminB12 and food aid program should be re-inforced for all pregnant women.
Cellular Influx and Cytotoxicity of Oxaliplatin Analogues ab 79.9 EURO Investigation conducted in the human ileocecal colorectal adenocarcinoma cell lines HCT-8 and HCT-8ox
High Quality Content by WIKIPEDIA articles! The VS-HCT series of mines are Italian plastic cased anti-tank mines that use Misznay Schardin effect warheads and have a dual seismic and magnetic fuze. The mines are no longer produced and differ in size and shape.The VS-HCT has a circular case with vertical ribbing. The mine can be either mechanically laid or laid by hand and incorporates both electronic and mechanical arming delays. The mines fuze has a battery powered seismic sensor which is used to detect approaching targets, and a magnetic sensor, which triggers the mine at the optimum point. Once triggered the mine fires a clearing charge, which clears up to 100 millimeters of earth that may have been laid on top of the mine. Once the clearing charge has been fired, the main charge is triggered. The main charge throws a heavy metal slug upwards, which is capable of penetrating up to 175 millimeters of armour.
This research work was carried out to establish scientific based evidence on folkloric usage and knowledge. The aim of this work is Pharmacological evaluation of Melochia corchorifolia leaves (folkloric) for antioxidant, diuretic, antiurolithiatic, anticancer, antibacterial and anthelmintic activities. In this regard, Melochia corchorifolia leaves were extracted with chloroform and ethylalcohol. The obtained extracts were used for pharmacological activities, the antioxidant activity results, the extracts and its Phenolic content shows good averting activity. Diuretic and saluretic activity is due to altering renal sodium excretion.The antiurolithiatic activity may be due to the presence of secondary metabolites of the extract.The extracts produced good anticancer activity on HCT-116 cell line and MCF-07 cancer cell lines. The chloroform extracts of Melochia corchorifolia shows good antibacterial and anthelmintic activities. So it was concluded that this research work supports the folkloric usage of this herbal drug (Melochia corchorifolia) is a potential source for treating various ailments.
HIV&AIDS Counselling and Testing (HCT) services have for long been seen as an intervention towards HIV prevention. The major objective of this study is to establish the Knowledge and Attitudes of youth towards HCT services in Lira district, Uganda. The study tried to establish the knowledge of the youth on HIV&AIDS and HCT services, to identify the attitudes of the youth towards HCT services and to find out the strategies for improving HCT uptake among the youth. A cross section study design was used to collect quantitative and qualitative data from 112 youth. Quantitative data was collected by the use of semi-structure questionnaire on youth between 15-24 years while qualitative data was obtained using key informant interviews and focus group discussions. The findings show that most youth are knowledgeable about HIV&AIDS and HCT services because they can identify modes of HIV transmission and where to obtain HCT services. Youth in school were more likely to have heard about HIV and AIDS compared to the youth out of school. The study shows that youth have both positive and negative attitudes towards HCT services.
Die zeitgemäße Version des 500/5 'President' Basses der 1950er und 1960er. Im Gegensatz zu den anderen Contemporary Modellen hat dieser Club Bass einen 16.5" breiten Vollresonanz-Korpus. Die beiden originalen Höfner 'Black Bar' Tonabnehmer ermöglichen zusammen mit dem traditionellen Höfner 'Control Panel' (= Reglereinheit) den legendären Sound der 60er. · Korpusform: Retro Style · Korpusmaterial: Ahorn (Maple) · Decke: Fichte (Spruce) · Bauart: Semi-Hollowbody · Farbe: 2-Tone Sunburst · Halskonstruktion: eingeleimt · Mensur: Shortscale · Mensur: 30,5" (77,47 cm) · Hals: 3-tlg. Ahorn/Buche · Griffbrett: Palisander (Rosewood) · Bundanzahl: 22 · Griffbrett Einlagen: Dot Inlays · Saitenanzahl: 4-saitig · Pickup Wahlschalter: Schiebeschalter · Hardware: Nickel · Besonderheit(en): Hollowbody · Produktionsland: China E-Bass
The human immunodeficiency virus (HIV) has created an enormous challenge worldwide. Since its recognition, HIV has infected close to 70 million people, and more than 30 million have died due to acquired immunodeficiency syndrome (AIDS). In 2009, around 400,000 children aged under 15 became infected with HIV. Almost all of these infections occurred in low and middle-income countries, and more than 90% were the result of mother-to-child transmission (MTCT) during pregnancy, labour and delivery, or breastfeeding. Without intervention, there is a 20-45% chance that a baby born to an HIV-infected mother will become infected. Prevention of Mother To Child Transmission (PMTCT) of HIV is a key component of overall HIV prevention efforts and represents a critical opportunity for stemming the tide of the HIV epidemic. With current intervention, the risk of mother to child transmission can be reduced to less than 5%. Therefore, transmission of HIV from a pregnant woman to her infant is preventable. HIV Counseling and Testing (HCT) is an important entry point for HIV prevention and for early access to treatment, care and support.
In pregnancy the increased blood supply includes a 45% to 50% increase in plasma volume and 20% to 30% increase in red blood cells. Since these percentages are not equal, the subsequent hemoglobin (HGB)/hematocrit (HCT) will reflect a normal physiologic anemia of pregnancy. The HCT will appear to fall as the volume increases more than the packed cell count. White blood cell (WBC) counts, especially neutrophils, increase naturally during pregnancy.There is also a rise in the number of platelets from 180 x 10^9 to over 300 x 10^9 during pregnancy. Although all women experience some expected changes in clotting, there are defective clotting conditions that greatly affect the outcomes of some pregnancies.
This comprehensive book thoroughly addresses all aspects of health care transition of adolescents and young adults with chronic illness or disability, and includes the framework, tools and case-based examples needed to develop and evaluate a Health Care Transition (HCT) planning program that can be implemented regardless of a patient's disease or disability. Health Care Transition: Building a Program for Adolescents and Young Adults with Chronic Illness and Disability is a uniquely inclusive resource, incorporating youth/young adult, caregiver, and pediatric and adult provider voices and perspectives.Part I of the book opens by defining Health Care Transition, describing the urgent need for comprehensive transition planning, barriers to HCT and then offering a framework for developing and evaluating health care transition programs. Part II focuses on the anatomic and neuro-chemical changes that occur in the brain during adolescence and young adulthood, and how they affect function and behavior. Part III covers the perspectives of important participants in the HCT transition process - youth and young adults, caregivers, and both pediatric and adult providers. Each chapter in Part IV addresses a unique aspect of developing HCT programs. Part V explores various examples of successful transition from the perspective of five key participants in the transition process - patients, caregivers, pediatric providers, adult providers and third party payers. Related financial matters are covered in part VI, while Part VII explores special issues such as HCT and the medical home, international perspectives, and potential legal issues. Models of HCT programs are presented in Part VIII, utilizing an example case study.Representing perspectives from over 75 authors and more than 100 medical centers in North America and Europe, Health Care Transition: Building a Program for Adolescents and Young Adults with Chronic Illness and Disability is an ideal resource for any clinician, policy maker, caregiver, or hospitalist working with youth in transition.
Master Thesis in Applied Chemical Science, it Includes studing 27 different olive tree cultivars leaves ethanolic extracts in terms of their total phenolic content, antioxidant activity, toxicity to brine shrimps, anticancer activity (cell cycle analysis, cell death assay, MTT test), safety on normal cells (MTT test), LC-MS anlysis and quntification for some of their chemical constituents like Oleuropein, Oleanolic acid, Erythrodiol with its isomer Uvaol, Quercetin, Rutin. investigation of the presence of Resveratrol and Salicin qualitatively and quantitatively using LC-MS anlysis , studing the correlation between each constituent concentration in the extracts and their toxicity to brine shrimps also their correlation with anticancer activity on 7 cancer cell lines (MCF- 7, MDA-MB-231, C32, MV3, SW480, SW620, HCT-116), LC- MS(-)ESI,(+)APCI finger print of the most potent anticancer extracts.
Phytic acid is extensively known for its anti-nutritional properties. The wide notion about this compound is to be changed totally. Hence, the study is focused on bringing out its potential as a drug molecule. The knowledge gained by understanding the binding efficacy of phytic acid and its analogues can help in the development of a new lead molecule. The antibacterial studies and cell line studies using HCT-15 confirmed the drugability. The potency of cancer drug targets can also be screened using docking studies. The entire work can bring out an effective lead molecule which maybe subjected to clinical trials. It is hypothesized that phytic acid and its derivatives having one or more of the following chemical functional groups such as the number of phosphates, phospho-diesters, charged phosphates, halogens, heavy atoms, steriocentred atoms, hydoxyl groups, hydrogen donors and acceptors, have an effect on the binding affinity towards the selected targets. Although, there are few reports to suggest the anticancer activity of PA, there is no work on its structure-activity relationship.